The transformation conditions optimized were, pricking of the apical and intercotyledonary region of the seedling axes of two-day old germinating seedlings with a sewing needle, infection with Agrobacterium (LBA4404/pKIWI105 carrying uid A and npt II genes) in Winans’ AB medium that was added with wounded tobacco leaf extract, co-cultivation in the same medium for 1h and transfer of seedlings to soilrite for further growth and hardening and subsequent transfer of seedlings to soil in pots in the greenhouse. The plumular and intercotyledonary meristems of the seedling axes are targeted for transformation. The protocol involves raising of whole plant transformants (T0 plants) directly from Agrobacterium-infected young seedlings. This article presents a non-tissue culture-based method of generating transgenic pigeon pea (Cajanus cajan (L.) Millisp.) plants using Agrobacterium-Ti plasmid-mediated transformation system. The results demonstrate the potential of a PR protein from a heterologous source in developing fungal disease-resistant safflower.ĭevelopment of transgenics in pigeon pea remains dogged by poor plant regeneration in vitro from transformed tissues and low frequency transformation protocols. Overall the method resulted in a transformation efficiency of 7.72% on analysis of T 1 plants. carthami, these plants showed not only a reduction in the number of spots but also a delay in the onset of disease. PCR was used to confirm stable integration of the chitinase gene in transgenic safflower plants. 'A-1' and its resistance towards Alternaria carthami. We report in this paper, overexpression of a rice chitinase in transgenic safflower cv. ![]() The PR protein chitinase hydrolyses a major cell wall component, chitin of pathogenic fungi and acts as a plant defense barrier. Overexpression of pathogenesis-related (PR) proteins leads to increased resistance to pathogenic fungi in several crops. Yield losses due to fungal diseases are enormous in the cultivation of this crop. ![]() ![]() Safflower (Carthamus tinctorius L.) is an important oilseed crop of semi-arid regions.
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